![]() ![]() The principle and detailed analysis method by DLS measurement will be described in this section. Dynamic light scattering (DLS), which is also known as photon correlation spectroscopy ( PCS) or quasi-elastic light scattering (QLS), is a spectroscopy method used in the fields of chemistry, biochemistry, and physics to determine the size distribution of particles (polymers, proteins, colloids, etc.) in solution or suspension. Due to its nature, other equipment must be used to obtain detailed information such as the shape. Multiple-scattering effects of light scattering by particles are treated by radiative transfer techniques (see, e.g. It should also be noted that the obtained size includes a solvated layer around the particles. The relation between the speed of the particles and the particle size is given by the Stokes-Einstein equation (Equation 1). ![]() However, data analysis of DLS is based on the assumption that the particles are uniform and spherical. Since the size information of particles, molecular aggregates, and emulsion droplet is extremely important, DLS is widely preferred by many colloid researchers. Dynamic light scattering (DLS) uses the above principle and provides size information of a wide range of particles easily and accurately. ![]() By obtaining the autocorrelation function of the observed fluctuation, the particle size and distribution can be obtained. Since this Brownian motion depends on the velocity of the particle size, the size can be estimated by observing the fluctuation corresponding to this velocity. Particles dispersed in a solution are constantly moving in Brownian motion. Dynamic light scattering (DLS) or photon correlation spectroscopy is one of the most popular light scattering techniques that measuring the particle size. ![]()
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